Synthèse in vitro de la β-galactosidase et de la phosphatase alcaline par les fractions subcellalaires particulées isolées à partir d'escherichia coli

Abstract Treatment of the protoplasts of Escherichia coli by digitonin enables to separate particulate fractions. These fractions are shown to be able to perform the synthesis in vitro of specific proteins, such as β-galactosidase and alcaline phosphatase. The purification and the isolation of the enzymes synthesized from radioactive amino acids leads to the conclusion that synthesis has been accomplished in vitro . DNA plays a specific role. A stimulation is obtained by the enzymes synthesising RNA. The nature of synthesis in vitro is discussed.