DNA Barcodes for certain endangered Indian Tylophora species

tRNA Leu (UAA) (trnL) intron sequences have been used as a molecular marker for differentiating plant species and to assess phylogenetic relationships among three closely related Tylophora species. Mutation events were calculated based on the sequences generated. Nucleotide substitutions were 31% and indels, 69%. Transversions of G↔C were dominant as compared to A↔C and A↔G. AT repeats of variable length were observed in all the three species. Further three repeat clusters with a p value of 1.58x10-5 were observed in all the three species. cpDNA polymorphism can provide significant insight into the evolution of the species and its genetic structure.