Role of Rho-kinase in vascular hyporeactivity following hemorrhagic shock in rats
2006
Objective To observe the role of Rho-kinase in vascular hyporeactivity following hemorrhagic shock(HS) in rats.Methods The superior mesenteric artery(SMA) from hemorrhagic shock rats was adopted to assay the vascular reactivity,and calcium sensitivity via observing the contraction initiated by norepinephrine(NE) and Ca~(2+) under depolarizing conditions(120mmol/L K~(+)) with isolated organ perfusion system.At the same time,Rho-kinase activity in SMA was measured.The relationship among the vascular reactivity,calcium sensitivity and Rho-kinase activity was analyzed;meanwhile,the effect of the Ang-Ⅱ and Y-27632 of Rho-kinase activity regulating agents on vascular reactivity was also observed.Results As compared with the control group,the cumulative dose-response curves of SMA to NE and Ca~(2+) at early period of shock shifted to the left,the maximal contractions(Emax) of NE and Ca~(2+) was increased significantly(P0.05 or P0.01).But the cumulative dose-response curves of SMA to NE and Ca~(2+) on 1,2h after shock shifted to the right,the maximal contractions(Emax) of NE and Ca~(2+) were significantly decreased(P0.05 or P0.01).Rho-kinase activity was significantly increased in the period of early shock(P0.05),but significantly decreased on 1,2h after shock(P0.05 or P0.01).Ang-Ⅱ increased the contractile response of SMA to NE and Ca~(2+) on 2h after shock,which made the cumulative dose-response curve of NE and Ca~(2+) shift to the left(P0.05 or P0.01);Y-27632 decreased the contractile response of SMA to NE and Ca~(2+),which made the cumulative dose-response curves of NE and Ca~(2+) shift to the right(P(0.05) or P0.01).Conclusion Rho-kinase can regulate vascular reactivity via!calcium sensitivity regulation.The changes of vascular reactivity following hemorrhagic shock was closely associated with the changes of Rho-kinase activity in SMA.Rho-kinase plays an important role in vascular hyporeactivity following hemorrhagic shock.
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