Chromatographic Studies on Protein Metabolism in Rice Plant from Seedtime to Harvest

Of the nitrogen metabolism in rice-plants, it is too well known to introduce that many con-tributions to these problems have already been published in Japan. But, on the contrary, few papers appeared from the view point of the recent science related to the protein metabolism. Therefore, the present authors have attempted further chromatographic studies on protein metabolism in rice plants prepared with the water culture by KASUGAI's method. At first, free amino acids were separated from various parts of 'rice-plants at different stages from seedtime to harvest, and the residual parts were susequently treated with several kinds of solution such as cold water, 10% NaCl, 75% ethanol and 0.2% NaOH. Each fraction was decomposed with 25% hydrolytic acid and the composition of the hydrolyzates thus obtained was tested by the ordinary chromatographic process. Results may be briefly summarized shown in Table 1_??_7. From the results of experiments, the varieties and contents of amino acids in protein meta-bolism of rice plants was confirmed at every stages from seedtime to harvest.