Interaction between thyrotropin and prostaglandins E and plasma membranes of human isolated thyrocytes

Thyrotropin (TTH) and prostaglandin E2 (PGE2) interaction was studied, using the euthyroid paranodal tissue (isolated by sub-surgery) of the thyroid in patients with nodose goiter. Two types of specific binding, differing by the affinity and ligand-bound capacity, were discovered both for TTH and PGE2. The appropriate kinetic characteristics were analysed. The data concerning a possible PGE2 receptor localization on the external side of the thyrocyte plasma membrane were obtained by means of affinity chromatography. A precise relationship between specific TTH-PGE2 binding and human thyroid cells was revealed.