Effects of Insulin Pump on Endothelia-dependant Flow-mediated Vasodilatation and Serum 3-nitrotyrosine in Patients with Newly Diagnosed Type 2 Diabetes Mellitus

Objective To investigate the effects of short-term insulin pump therapy on flow-mediated dilatation(FMD) and serum 3-NT concentration in patients with newly diagnosed type 2 diabetes mellitus.Methods 40 patients with newly diagnosed type 2 diabetes mellitus were selected as DM group and 30 age-and sex-matched healthy people were selected as NC group.In the two groups,blood sugar,blood fat and glycosylated hemoglobin were tested,FMD was tested by high resolution ultrasound detection,and serum 3-NT concentration was tested by enzyme linked immunosorbent assay(ELISA) detection.Besides,fasting insulin was measured and HOMA-IR was calculated in DM group.After intensive therapy by insulin pump for 2 weeks,FMD and serum 3-NT concentration were reexamined in DM group.Results(1) Compared with the NC group,the T2DM group had significantly lower HDL-C and FMD,and higher glycosylated hemoglobin,blood glucose and blood fat as well as serum 3-NT concentration(P0.05).(2)After two-week insulin pump therapy,FMD in DM group increased from(4.54±1.42)% to(7.20±2.59)%(P0.01),serum 3-NT concentration decreased from(118±13)nmol/L to(112±11)nmol/L(P0.01).The difference was statistically significant(P0.01).(3) Correlation analysis showed that 3-NT level had positive correlation with glycosylated hemoglobin and fasting plasma glucose but negative correlation with FMD(P0.01).Conclusion Intensive treatment by insulin pump can increase FMD and decrease 3-NT concentration in patients with newly diagnosed type 2 diabetes mellitus.