Lysobacter rhizophilus sp. nov., isolated from rhizosphere soil of mugunghwa, the national flower of South Korea
2016
A Gram-staining-negative, aerobic, motile, rod-shaped, catalase- and oxidase-negative strain with one polar flagellum, designated THG-YS3.6T, was isolated from rhizosphere soil of a mugunghwa flower collected from Kyung Hee University, Yongin, South Korea. Growth occurred at 10–37 °C (optimum 25–30 °C), at pH 6–8 (optimum 7.0) and with 0–2.0 % NaCl (optimum 1 %). The isoprenoid quinone was ubiquinone-8 (Q–8). The major cellular fatty acids were iso-C11
: 0, iso-C11 : 0 3-OH, iso-C15 : 0, iso-C16 : 0, C16 : 1ω7c alcohol, C16 : 0, iso-C17 : 0 and summed feature 9 (iso-C17 : 1ω9c and/or C16 : 0 10-methyl). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, one unknown phospholipid, one unknown lipid and three unknown aminolipids. The DNA G+C content of strain THG-YS3.6T was 65.3 mol%. Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-YS3.6T were identified as
Lysobacter yangpyeongensis
KACC 11407T (98.7 %),
Lysobacter oryzae
KCTC 22249T (98.0 %),
Lysobacter niabensis
KACC 11587T (97.6 %) and
Lysobacter terrae
KACC 17646T (97.1 %). The DNA–DNA relatedness values between strain THG-YS3.6T and
L. yangpyeongensis
KACC 11407T,
L. oryzae
KCTC 22249T,
L. niabensis
KACC 11587T and
L. terrae
KACC 17646T were 53.8±1.0 %, 12.9±1.2 %, 10.9±0.6 % and 7.0±1.9 %, respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA–DNA hybridization data, strain THG-YS3.6T represents a novel species of the genus
Lysobacter
, for which the name
Lysobacter rhizophilus
sp. nov. is proposed. The type strain is THG-YS3.6T (=KCTC 52082T=CCTCC AB 2015358T).
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