Lincosamide resistance mediated by lnu(C) (L phenotype) in a Streptococcus anginosus clinical isolate

Methods: The strain UCN93 was recovered from neonatal specimens and from the mother’s vaginal swab. Identification was confirmed by sequencing of thesodA gene. Antimicrobial susceptibility testing was carried out by the disc diffusion method, while MICs were determined using the agar dilution method. Screening for lnu(A), lnu(B), lnu(C) and lnu(D) genes was performed by PCR. Genetic environment and support were determined by thermal asymmetric interlaced PCR and PCR mapping. The transfer of lincomycin resistance was also attempted by conjugation. Results: UCN93 was unambiguously identified asStreptococcusanginosus. It was susceptible to all tested antibiotics, except lincomycin (MIC, 8 mg/L) and tetracycline (2 mg/L). Thelnu(C) gene was found to be responsible for the L phenotype. It was shown that lnu(C) was associated with a gene coding for a transposase within a structure similar to the transposon MTnSag1, described once in Streptococcus agalactiae. Since MTnSag1 was found to be mobilized by Tn916 and S. anginosus UCN93 harboured a Tn916 transposon, several attempts at transfer were performed but they all failed. Thelnu(C)-containing genetic element was inserted into a chromosomal intergenic sequence of S. anginosus. Conclusions: Since lnu(C) has been detected in only one S. agalactiae clinical isolate so far, this is its second description among clinically relevant streptococci.