INTERNATIONAL JOURNAL OF PHARMACY & LIFE SCIENCES Media optimization for lipase from Halomonas salina, a moderately halophilic bactrium isolated from excreta of wild Ass.

Twenty-four moderate halophiles were isolated from excreta of wild Ass after enrichment in complete me dia broth and halophilic broth. All the isolates were Gram’s positive and non-capsulated. Further, isolates were screened for extracellular lipase secretion on solid media conta ining tributyrin as carbon source. Halomonas salina was the potential lipase producer identified on the basis o f 16s r-RNA sequencing and further optimized for li pase secretion in liquid media. Maximum lipase secretion was after 264 hours of incubation i.e. during stationary pha se. Isolates were found to secrete maximum lipase at pH 5, 14% NaCl, 30- 40˚C temperature and 3% substrate concentration.