Ileal interposition for the treatment of diabetes in rats: repercussion on beta cells mass Tratamento do diabetes pela interposição ileal em ratos: Repercussão na massa de células beta

Purpose: Research with the aim of studying the effect of ileum interposition in hyperglycemia and pancreatic beta cell mass in nonobese diabetic rats. Methods: We used 18 Wistar rats randomly divided into 3 groups of 6 each. A non-diabetic control group, a diabetic control group, and diabetic with ileal interposition group. Induction of diabetes was underwent with streptozotocin. The beta cell mass was quantified by an indirect method by dividing the serum C-peptide (ng/mL) for blood glucose (mg / dL). We used variance (ANOVA) and Tukey's test for analysis of specific differences, considering the significance p <0.05. Results: The blood glucose of diabetic animals with ileal interposition was significantly lower (94.5 ± 5.6mg/dL) than in diabetic control rats (245 ± 4.8 mg / dL) (p <0.05). The C-peptide levels showed significantly higher in diabetes interposition group (0.58 ± 0.06 ng / mL) than in control diabetes group (0.42 ± 0.03 ng / mL), suggesting a greater response to pancreatic stimulation in that group (p <0.05). The ratio of C-peptide and glucose levels showed a higher beta cells mass in diabetic interposition animals (0.61) than in control diabetes (0.004), and the difference was significant (p<0.05). Conclusion: The results showed that the ileal interposition contributed to the reduction of blood glucose and to increase the mass of functioning beta cells in diabetic rats.