Method for rejecting B2M (beta 2-microglobulin) gene segment
2015
The invention discloses a method for rejecting a B2M (beta 2-microglobulin) gene segment. The method comprises steps as follows: (1) constructing a CRISPR/Cas9 vector system; (2) introducing a target gene into a recipient cell by the aid of the constructed CRISPR/Cas9 vector system, and rejecting the B2M gene segment; (3) detecting and identifying the transfected recipient cell. The method is a new genetic engineering method for rejecting a human cell gene in a targeted manner. According to the method, a baculovirus vector is used as a transfer system, a specific CRISPR/Cas9 component with a B2M gene rejected is efficiently expressed in a human cell, B2M gene expression is effectively blocked, and expression of a cell surface type-I HLA (human leukocyte antigen) molecule is reduced, so that a low-immunogenicity human cell is produced.
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