PERIPHERAL BLOOD LYMPHOCYTES ADENOSINE TRIPHOSPHATE AVAILABILITY AMONG THE RESIDENTS OF THE NORTHERN EUROPEAN RUSSIA

Metabolic pathways participating in adenosine triphosphate (ATP) synthesis play an important role in lymphocytes normal development and functioning. Energetic metabolism has an influence on differentiation, proliferation and fate of lymphocytes. The aim of the study is to define ATP availability of peripheral blood lymphocytes and the level of mitochondrial metabolism regulator sirtuin 3 (SIRT3) in healthy northerners. Methods. 106 volunteers, habitants of Arkhangelsk (76 women and 30 men, 21-63 years old) were surveyed. Amounts of immunocompetent cells with receptors (CD3, CD4, CD8, CD10, CD16, CD71, CD23, CD25, HLA DR, CD95) were determined by indirect immunoperoxidase method, ATP concentration in lymphocytes by bioluminescent method, and additionally SIRT3 (23 volunteers) by enzyme immunoassay. Two groups were selected using "k-means" cluster analysis which differed statistically significantly in all studied parameters. Results. It was found that 78 % of the surveyed participants were in the group where ATP concentration was 0,95 (0,487) mcmol/106 cells, in other group with 22 % participants it was 3,71 (1,319) mcmol/106 cells, р < 0,0001. Difference in relative amounts of particular cell phenotypes was revealed in the mentioned groups: in the group with lower ATP levels the higher was percentage of CD95+, CD23+ and HLA DR cells. In the group with higher ATP levels the higher was percentage of CD3+, CD4+, CD8+, CD71+ cells and shift of CD10/CD95 and CD4/CD23 ratios was also detected in favor of CD10+ и CD4+ cells with high levels of metabolic activity. Difference of ATP levels was omni-directional with SIRT3, regulator of mitochondrial metabolism, level with concentrations 0,15 (0,039) and 0,39 (0,198) pg/106 cells, р = 0,0097. Conclusion. Intersectionality of ATP availability and reactivity of T cells was identified. It appears to be promising to determine the level of ATP for evaluation of lymphocytes functioning.