Analytical method for structures of microbial communities in composite filler in tower-type earthworm ecological filter

The invention relates to an analytical method for compositions of microbial communities in composite fillers (soil, gravels and broken bluestones) in a tower-type earthworm ecological filter. The analytical method can be used for better reflecting the diversity and dynamic variation of microbial populations and specifically comprises the following steps of: (1). respectively taking samples in different filler layers of a tower-type earthworm ecological filter for preprocessing; (2) extracting the total DNAs (Deoxyribose Nucleic Acids) of genomes by using a MOBIO DNA extraction kit; (3) carrying out nest type PCR (Polymerase Chain Reaction) amplification on the DNAs of the samples by using a design primer of a 16S RNA V3 region; (4) analyzing PCR amplification products by using a denaturing gradient gel electrophoresis (DGGE) technology, and analyzing an obtained DGGE atlas by using Quantity One software; (5) cutting and recycling characteristic DGGE bands, carrying out sequencing analysis on the DNAs of the samples after carrying out PCR amplification again, and judging the variety of microorganisms.