Ageing influences the relationship of circulating miR-33a and miR - 33b levels with insulin resistance and adiposity

Objective:The identification of circulating microRNAs related to abnormal metabolic function may be useful in the context of ageing, adiposity and insulin resistance. The miR-33a/b has been shown to control the expression of genes involved in fatty acid biosynthesis, impaired metabolism and insulin resistance. In this study, we aimed to identify differences in circulating miR-33a/b levels according to age-related metabolic impairment and increased adiposity.Methods:This study included 80 individuals (30.2% with obesity, 70% females) classified according to insulin resistance (Stern’s criteria) and age [young (20–39 years) and senior (40–59 years)]. Body fat was evaluated using bioelectrical impedance, biochemical markers by colorimetric, enzymatic and immuno-turbidimetry methods. TaqMan measures of circulating miR-33a and miR-33b with quantitative reverse transcription polymerase chain reaction in serum were assessed in association with clinical outputs.Results:Circulating miR-33a and miR-33b levels showe...