High quality bisulfite sequencing using nanogram amounts of genomic DNA
2013
Multiple methods have been developed to decipher the genome-scale DNA methylation (Methylome). Bisulfite sequencing (BS-seq), which combines sodium bisulfite conversion with high throughput sequencing, can measure DNA methylation at single-nucleotide resolutions. However, it normally needs large amounts of genomic DNA (5-10μg) to start with, thus creating an obstacle for it to be widely used. Here we optimized the normal BS-seq method for generating high quality bisulfite sequencing on whole genomes using nanogram DNA (MBS-seq). Systematic comparison the whole genome methylation study based on minute amount of genomic DNA through next generation sequencing technology.
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