Effect of CaMK II expression on apoptosis of rat hepatocytes BRL-3A

Objective To investigate the effect of CaMKⅡ expression on apoptosis of rat hepatocytes BRL-3A. Methods Rat BRL-3A cells were stable passage were cultured. The CaMKⅡγ protein (LV-CaMKⅡγ group) and CaMKⅡγshRNA (shRNA group) lentiviral expression systems were constructed. The corresponding blank vectors (LV-NC group and shRNA-NC group) and normal saline (CON group) were perfused into the control groups. The expression levels of CaMKⅡ, Cyt C and AIF proteins were detected by Western blotting, and the apoptosis rate of BRL-3A cells was measured by Tunel method. Results The protein expression of CaMKⅡ, Cyt C and AIF in LV-CaMKⅡγ group was significantly higher than that in CON group (P 0.05). At the same time point, the apoptosis rate of hepatocytes in LV-CaMKⅡγ group was significantly higher than that in CON group (P 0.05). Conclusion The specific CaMKⅡ signaling pathway can inhibit the apoptosis of BRL-3A cells, while the enhanced CaMKⅡ signaling pathway promotes the apoptosis of BRL-3A cells. Key words: CaMKⅡ; Rat hepatocytes; BRL-3A; Lentivirus; Cell apoptosis