Compositions to provide multiple herbicide tolerance and methods of use thereof

Methods and compositions relating to improved plants that are tolerant to more than one herbicide are provided. Particularly, the invention provides plants that are tolerant to glyphosate and are tolerant to at least one ALS inhibitor, and methods of use thereof. Tolerant plants glyphosate / ALS inhibitor comprising a polynucleotide encoding a polypeptide that confers tolerance to glyphosate and a polynucleotide encoding a polypeptide tolerant to ALS inhibitor. In specific embodiments, a plant of the invention expresses a GAT polypeptide and an HRA polypeptide. Methods are provided to control weeds, improve plant yield, and increase transformation efficiency.