METABOLISM OF AMINOACYL-P-NITROANILIDES BY RAT MAMMARY TISSUE

Abstract We have examined the metabolism of aminoacyl- p -nitroanilides by rat mammary tissue isolated from rats during late pregnancy, peak lactation and late lactation. The rate of hydrolysis depended upon the chemical nature of the aminoacyl- p -nitroanilide compound and the physiological state of the donor animals. Thus, mammary tissue isolated from rats during late pregnancy and peak lactation hydrolysed aminoacyl- p -nitroanilides in the order l -met- p -nitroanilide= l -leu- p -nitroanilide> l -lys- p -nitroanilide>γ-glu- p -nitroanilide. The order of activity was the same for mammary tissue taken from rats during late lactation except that l -lys- p -nitroanilide was hydrolysed at the same rate as the neutral aminoacyl- p -nitroanilides. Mammary tissue from peak lactating rats also hydrolysed α- l -glu- p -nitroanilide and α- l -asp- p -nitroanilide but to a lesser extent than the other compounds tested. The anionic aminoacyl- p -nitroanilides were able to trans -stimulate d -aspartate efflux from mammary tissue explants and the perfused mammary gland via the high-affinity anionic amino acid carrier. The clearance of gly- l -phe by the perfused mammary gland was markedly inhibited by l -phe. The results suggest that mammary tissue expresses a variety of dipeptidases at the basolateral aspect of the mammary epithelium which are capable of hydrolysing peptides extracellularly. These enzymes may be important for providing amino acids for milk protein synthesis and/or inactivating signal peptides.