265 Pilot trial to evaluate the effect of vitamin D on melanocyte biomarkers

264 Topical treatments improved periorbital aging J Namkoong, B Cook, D Kern and HE Knaggs Global R&D, Nu Skin Enterprises, Inc., Provo, UT Facial aging is manifested by different symptoms. One area people pay more attention to and use additional cosmetic products to keep healthier is the eye area e the periorbital region. There are multiple reasons why the periorbital region manifests an unhealthy and aged look. The skin in the eye area is thinner than other parts of face, and repetitive muscle movements and UV exposures lead to skin aging. Periorbital aging includes eyelid drooping, dark circles, under eye bags, or fine lines/wrinkles. Topical and mechanical treatments to improve periorbital aging is challenging due to the proximity to eyes. Hypersensitivity to treatment options are common occurrence in the eyes. In order to improve the skin in the eye area, topical ingredients were evaluated for their potential to modulate different skin aging target genes, such as improving skin structures, hydration, anti-inflammation, antioxidant capacity and cell turnover, using normal human skin equivalent cultures. Some interesting finding includes modulation of Prostaglandin E2 (PGE2). Induction of cellular stress with 5mM or 10mM hydrogen peroxide increased PGE2 expression, which would increase inflammation. In addition, PGE2 increase has been suggested in the skin of elderly and inhibition of PGE2 resulted in collagen production in skin organ culture. Experimental ingredients were able to suppress that H2O2-induced PGE2 expression, similar to ascorbic acid. After evaluating ingredients using in vitro models, a specific formula was developed to target clinical signs of periorbital skin aging. Prior to running a clinical study, the formula was evaluated for safety, due to potential hypersensitivity. This eye formulation did not trigger any safety concerns during in vitro evaluations and non-ocular evaluations. The formula was further assessed for safety during the efficacy testing by both dermatological and ophthalmologic evaluations. This formula improved periorbital aging in 50% of subjects with sagging skin, eyelid firmness and puffiness after 12 weeks of application by dermatological evaluations. 265 Pilot trial to evaluate the effect of vitamin D on melanocyte biomarkers E Anderson, M Rezaee, I Bailey-Healy, K Sarin and J Tang 1 School of Medicine, Stanford University, Stanford, CA and 2 Dermatology, Stanford University, Stanford, CA The role of vitamin D for cancer prevention is an expanding area of interest. In clinical and epidemiologic studies, vitamin D has been shown to predict incidence and severity of melanoma skin cancer. Although the role of vitamin D in melanocyte differentiation and melanoma carcinogenesis has been described, a clear mechanism is yet to be established. Previous studies have suggested that vitamin D supplementation and increased serum vitamin D levels are associated with decreased melanoma incidence. We sought to determine the effect of oral vitamin D supplementation on gene expression within melanocytic nevi of women previously diagnosed with non-melanoma skin cancer. A total of 24 healthy women were included in this randomized placebo-controlled study. Half of the cohort received 4,000 IU oral vitamin D3 daily for 8 weeks. Skin checks were performed at baseline and 8 week follow-up, and nevi were biopsied at both time points. RNAwas collected from these nevi for gene expression profiling to identify activity in signaling pathways associated with vitamin D treatment. Women who received vitamin D supplementation experienced a significant increase in vitamin D levels at follow-up (p 1⁄4 0.0001) while women who received placebo did not (p 1⁄4 0.50). Gene expression analysis of melanocytic nevi revealed 1048 genes that were significantly differentially expressed between vitamin D-treated and placebo-controlled women. Ingenuity Pathway Analysis indicated that the most significantly differentiated molecular signaling pathways were related to immune system signaling, including primary immunodeficiency signaling (p 1⁄4 3.53 x 10), T cell receptor signaling (p 1⁄4 4.31 x 10), iCOS-iCOSL signaling in T helper cells (p 1⁄4 1.24 x 10), calcium-induced T lymphocyte apoptosis (p 1⁄4 7.52 x 10), and crosstalk between dendritic cells and natural killer cells (p 1⁄4 1.68 x 10). This finding suggests that increased immune surveillance could underlie the previously described association between vitamin D supplementation and decreased melanoma incidence.