Actividad esporicida de la solución electrolizada con ph neutro en hongos de importancia postcosecha

espanolEl agua electrolizada con pH neutro (7) es un novedoso agente antimicrobiano, que tiene efecto en una gran variedad de microorganismos, seguro para los seres humanos y el medio ambiente. Se determino la eficacia del agua electrolizada de super oxidacion con pH neutro (SES) en la reduccion de la germinacion de esporas y desarrollo del tubo germinativo en hongos de importancia postcosecha. Una suspension de 8 × 10 7 esporas mL -1 de los hongos Botrytis cinerea aislado de zarzamora, Colletotrichum gloeosporioides aislado de mango, guayaba y lichi, Fusarium solani aislado de chile y estevia, Monilinia fructicola aislado de durazno, Penicillium digitatum aislado de limon mexicano y limon persa, Penicillium sp., aislado de papaya y Rhizopus stolonifer aislado de yaca y guanabana, estuvieron en contacto con la SES por 5 min a concentraciones de 3, 5, 6, 8, 18, 24, 27, 29, 36 y 43 ppm de cloro libre, y agua destilada esteril (testigo). Las esporas se sembraron en medio de cultivo papa dextrosa agar (Bioxon ® ); las evaluaciones se realizaron a las 24 y 48 horas posteriores a la siembra. La inhibicion de 100% en la germinacion de esporas y longitud del tubo germinativo de Botrytis se observo en el rango de concentracion de 18-43 ppm, Colletotrichum (6-43 ppm), Fusarium (6 - 43 ppm), Monilinia (8 y 24-43 ppm), Penicillium (18-43 ppm), Rhizopus aislado de guanabana (5-24 y 29-43 ppm) y Rhizopus de yaca (18-43 ppm). Los resultados sugieren que la SES podria ser utilizada como alternativa de control de hongos poscosecha. Palabras clave: Enfermedad, esporas, fruto, pudricion, patogeno. EnglishThe electroless water with neutral pH (7) is a novel antimicrobial agent, which has an effect on a large variety of microorganisms, safe for humans and the environment. The efficiency of electrolyzed super oxidation water with neutral pH (SES) in the reduction of spore germination and development of the germinative tube in fungi of postharvest importance was determined. A suspension of 8 × 10 7 mL -1 spores of the fungi Botrytis cinerea isolated from blackberry, Colletotrichum gloeosporioides isolated from mango, guava and lychee, Fusarium solani isolated from chili and stevia, Monilinia fructicola isolated from peach, Penicillium digitatum isolated from Mexican lemon and Persian lemon, Penicillium sp., papaya isolate and Rhizopus stolonifer isolated from yaca and soursop, were in contact with the SES for 5 min at concentrations of 3, 5, 6, 8, 18, 24, 27, 29, 36 and 43 ppm free chlorine, and sterile distilled water (control). Spores were seeded in culture medium potato dextrose agar (Bioxon ® ); the evaluations were carried out at 24 and 48 hours after sowing. The 100% inhibition of spore germination and Botrytis germ length was observed in the concentration range of 18-43 ppm, Colletotrichum (6-43 ppm), Fusarium (6-43 ppm), Monilinia (8 and 24-43 ppm), Penicillium (18-43 ppm), Rhizopus isolated from soursop (5-24 and 29-43 ppm) and Rhizopus of yaca (18-43 ppm). The results suggest that SES could be used as an alternative control of post-harvest fungi. Palabras clave: Disease, fruit, pathogen, rot, spores.