Analysis of Castanea mollissima germplasm resources by AFLP.

The genetic variation and genetic relationships of 90 Castanea mollissima cultivars were analyzed by the fluorescent-AFLP technique with 9 primer combinations.Among the 806 bands detected, 695 bands were polymorphic,and the high percentage of polymorphic loci(86.23%)revealed a high genetic diversity among the 90 C.mollissima cultivars.The UPGMA phenogram based on genetic identity clustered the 90 C.mollissima cultivars into 2 groups and 14 rows.The 9 pairs of AFLP primer combination used could distinguish all the tested cultivars,the highest ratio of identification is 93% and the lowest is 80%.