Isolation and bioactivity evaluation of two metabolites from the methanolic extract of Oroxylum indicum stem bark

Abstract Objective To isolate metabolites from the methanolic extract of Oroxylum indicum (O. indicum ) stem bark and test their bioactivities. Methods Silica gel column chromatography was employed to isolate bioactive metabolites. The isolated metabolites were confirmed by C18 column isocratic and gradient HPLC. The antioxidant activity of the isolated compounds was tested by DPPH radical scavenging assay and their cytotoxicity were tested on cancerous cells (MDA-MB-435S) and normal cells (WRL-68) by the XTT assay. Results HPLC confirmed the presence of single/pure metabolites. Bioactivity of the two isolated compounds confirmed that the Compound-1 has cytotoxic potential. The cytotoxicity of Compound-1 on cancer cells was found to be 1.5 times higher than in normal cells. Compound-2 on the other hand exhibited good antioxidant property. Conclusions The isolated compounds hold promise to serve as a medicine against diseases involving metabolic stress, genotoxicity/cytotoxicity and cancer.