Effects of Galectin-1 expression on the proliferation, migration, and invasion in the thyroid papillary carcinoma

Objective To observe the expression of galectin-1 in papillary thyroid carcinoma and explore its effect on proliferation, migration, and invasion in the papillary thyroid carcinoma. Methods 132 cases of thyroid surgery patients in our hospital as the research object, using the immune group of Galectin-1 and Galectin-3 expression was detected in tissue resection, analysis of two kinds of protein in the diagnosis of benign thyroid diseases and thyroid papillary carcinoma value. TCP-1 thyroid papillary carcinoma cell line was used as the research object to silence Galectin-1 gene expression, and to compare the proliferation, migration and invasion ability of TPC-1 cells after silence and silence Galectin-1. Results The sensitivity, specificity, positive likelihood ratio and negative likelihood ratio of Galectin-1 and Galectin-3 in diagnosing papillary thyroid carcinoma were 94.1%, 96.8%, 29.406 and 0.061, 94.7%, 81.9%, 5.199 and 0.072, respectively. The specificity of Galectin-1 in diagnosing papillary thyroid carcinoma was significantly higher than that in Galectin-3 (χ2=8.453, P=0.004). There was no significant difference between the two groups in the diagnosis of thyroid carcinoma (χ2=0.253, P=0.617). The A570 of untransfected TPC-1 cell line and transfected liposome TPC-1 cell line was significantly higher than that of Galectin-1 cell TPC-1 [untransfected TPC-1 cell line: t=2.470, 4.478, 8.860, 17.539, P=0.019, 0.000, 0.000, 0.000; transfected liposome TPC-1 cell line: t=3.471, 3.527, 7.580, 20.270, P=0.001, 0.001, 0.000, 0.000]. When transfected with 12, 24, 36, 48 and 72 h. The maximum width of scratch line of untransfected TPC-1 cell line and only transfected liposome TPC-1 cell line was significantly lower than that of TPC-1 cells that knocked out Galectin-1 gene (P=0.000). The results of Transwell cell invasion test showed that the number of invasive cells in untransfected TPC-1 cell line and transfected liposome TPC-1 cell line was significantly higher than that in TPC-1 cell knockout Galectin-1 gene (P=0.000). Conclusion Galectin-1 can promote proliferation, migration and invasion of papillary thyroid carcinoma, and can be used as a good molecular marker for differentiating and diagnosing papillary thyroid carcinoma. Key words: Thyroid papillary carcinoma; Galectin-1; Proliferation; Migration; Invasion