NUCLEOTIDE SEQUENCE AND FUNCTIONAL ANALYSIS OF REGULATORY REGION OF THE LUMP AND THE LUX OPERON FROM PHOTOBACTERIUM LEIOGNATHI

Abstract The lumP gene is linked to the lux operon, but runs in the opposite direction in Photobacterium leiognathi PL741. The gene order of the lumP and the lux operon is -lumP-RR the P R - and P L -promoter might be competing for recognition by RNA polymerase to initiate transcription. The fact of the LumP responsible for the spectral blue shift in P. leiognathi implied that the lumP gene closedly linked to the lux operon is for coordinative regulation with the lux operon. In addition, the glucose repression on the P R -promoter system shows that the expression of the lux operon is regulated by cAMP-CRP induction in E. coli .