New shrimp IgE‐binding proteins involved in mite‐seafood cross‐reactivity

Scope: Shrimp is a seafood consumed worldwide and the main cause of severe allergenic reactionstocrustaceans.Seafoodallergyhasbeenrelatedtomitesensitization,mainlymediated by tropomyosin, but other proteins could be involved. The aim of the study was to identify new shrimp allergens implicated in mite-seafood cross-reactivity (CR) in two different climate populations: dry and humid climates. Methods and results: Shrimp and mite IgE-binding profiles of patients from continental dry and humid climates were analyzed by immunoblotting, and the most frequently recognized Solenocera melantho shrimp proteins were identified by MS as -actinin, -actin, fructose biphosphate aldolase, arginine kinase, sarcoplasmic calcium-binding protein, and ubiquitin. Using inhibition immunoblot assays, we demonstrate that tropomyosin and ubiquitin were responsible for mite-seafood CR from both climates; but also -actinin and arginine kinase are implicated in dry- and humid-climate populations, respectively. Reciprocal inhibition assays demonstratedthatmitesaretheprimarysensitizerinhumid-climate,asshrimpisincontinental dry-climate population. Conclusion: Severalnewshrimpallergenshavebeenidentifiedandshouldbeconsideredinthe diagnosis and treatment of shrimp allergy and mite-seafood CR. Differences in mite-seafood CR were founded to be based on the climate.