红外能谱测量法20 mg酸化胶囊13C-UBT的效能评价

Objective To investigate whether a 13C-urea breath test (13C-UBT), using a c-urea dose of less than routine 75-100 mg, and lower-priced infrared spectrometry, can preserve diagnostic efficacy for H. pylori infection in clinical practice. Methods A total of 133 consecutive dyspeptic patients, who received gastroendoscopy were enrolled the study. The status of H. pylori infection was confirmed by histology on gastric biopsies. Breath tests were carried out as following: after baseline breath samples were collected 5 min after oral administration of 100 mL of citric acid solution, then a capsule containing 20 mg 13C-urea was given to the patients, a further sample was collected 15 min later. The duplicate gas samples were collected for an analysis of any excess 13CO2/12CO2 ratio by infrared spectrometry and data over baseline (DOB) were acquired. Best cut-off values were determined by receiver operating characteristic (ROC) analysis and specific priority analysis, respectively. Results the median value of DOB of 13C-UBT for 59 H. pylori positive patients was 5.27±4.49.10 times higher than that of 74 H. pylori negative patients (-0.50±0.98, p＜0.05). The best cut-off value of DOB for the diagnosis established by ROC analysis was 0.4, resulting in a sensitivity of 91.5% (54/59), a specificity of 89.2% (66/74) and an accuracy of 90.2% (120/133). However, on the bases of specificity priority consideration, the best cut-off value of DOB was 2.0 with a sensitivity of 86.4% (51/59), a specificity of 100% (74/74) and a accuracy of 94.0% (125/133). Conclusion the results of this study suggested that 13C-UBT using a 13C-urea dose less than routine 75-100 mg and infrared spectrometry measurement would be feasibility, but 20 mg protocol using in the this study are still not satisfaction for the clinic practice.