Comprehensive analysis of the regulatory network of blue-light-regulated conidiation and hydrophobin production in Trichoderma guizhouense.

Conidia of Trichoderma guizhouense (Hypocreales, Ascomycota) are frequently applied to the production of biofertilizers and biocontrol agents. Conidiation of some Trichoderma species depends on blue light and the action of different blue light receptors. However, the interplay between different blue-light receptors in light signaling remained elusive. Here, we studied the functions of the blue light receptors BLR1 and ENV1, and the MAP kinase HOG1 in blue light signaling in T. guizhouense. We found that the BLR1 dominates light responses and ENV1 is responsible for photoadaptation. Genome-wide gene expression analyses revealed that 1615 genes, accounting for ~13.4% of the genes annotated in the genome, are blue-light regulated in T. guizhouense, and remarkably, these differentially expressed genes (DEGs) including 61 transcription factors (TFs). BLR1 and HOG1 are the core components of the light signaling network, which control 79.9% and 73.9% of the DEGs, respectively. In addition, the strict regulation of hydrophobin production by the blue light signaling network is impressive. Our study unravels the regulatory network based on the blue light receptors and the MAPK HOG pathway for conidiation, hydrophobin production and other processes in T. guizhouense. This article is protected by copyright. All rights reserved.