The effect of surgical resection on serum proteomic profiles in non-small cell lung cancer (NSCLC)

21143 Background: Surface-enhanced laser desorption/ionisation time-of-flight mass spectrometry (SELDI) was used to detect characteristic serum proteomic profiles that discriminate between Non-small Cell Lung Cancer (NSCLC) and non-cancer controls. The changes in these profiles in response to curative resection were examined to determine if they returned to “normal” as would conventional tumor markers. Methods: Serum samples were collected from 70 patients and 75 controls. Further samples were obtained from 25 of these patients following resection with curative intent. SELDI analyses using CM10 & IMAC ProteinChip arrays were undertaken. The Wilcoxon rank test was used to detect differentially expressed peaks. Results: A preliminary analysis using CM10 on 39 patients, 38 controls, and 17 follow-ups showed 19 differentially expressed peaks (p<0.01) but no evidence of return to normal after treatment. The IMAC chip analysis, on all samples, revealed 40 discriminatory peaks (p<0.01). These data were used to c...