Mitochondrial DNA maintenance in yeast requires a protein containing a region related to the GTP-binding domain of dynamin.

Nuclear gene products replicate and partition mitochondrial DNA (mtDNA) molecules in the yeast Saccbaromyces cerevisiae. However, few of the relevant genes have been identified. A mutation that results in temperature-sensitive loss of mtDNA identifies one of these genes, MGMl. Deletion of MGMl shows that aside from its role in the mitochondrion, the gene has no essential cellular function. The MGMl protein has a 200-amino-acid region that is highly related to a family of GTP-binding proteins of apparently diverse function that includes the microtubule-binding protein, dynamin DIOO. The temperature-sensitive strain partitions mtDNA molecules at the restrictive temperature, but a defect in mtDNA synthesis results in a reduction in the number of molecules per cell at each cell division. On the basis of the results of this study, we conclude that cells can partition single mitochondrial genomes, and that when a cell receives a single molecule at division it is able to restore the normal complement of multiple copies.