Separation echinocandins nucleus b

The present invention provides an echinocandin B nucleus separation, the method comprising the steps of: 1) filtration after the conversion solution comprising echinocandin B nucleus, and the filtrate was adjusted to pH 5-8, a large macroporous styrene nonpolar adsorption resin, eluting with water and methanol, and then the lower aqueous methanol echinocandin B nucleus wash concentrate; 2) step 1) was collected macroporous acrylic resin, then the aqueous acid echinocandin B nucleus concentrated under wash; 3) step 2) pH adjusted to 4-5 were collected, lyophilized and then concentrated to obtain echinocandin B nucleus. This method not only can well remove the pigment, and is suitable for large-scale industrial production, has a good commercial value. The method of the present invention is capable of echinocandin B nucleus final purity increased to 92%.