Role of anti-proliferation of 2-(3-carboxy-1-oxoprogy1)amino-2-deoxy-D-glucose in human esophageal cancer cells

Objective To investigate the effects of 2-(3carboxy-1-oxoprogy1)amino-2-deoxy-D-glucose(COPADG) on the proliferation and cell cycle of human esophageal cancer cells Eca-109 and TE-1.Methods In vitro experiments,MTT colorimetric assay was performed to determine the growth inhibitory rates of Eca-109 and TE-1 cells treated by COPADG.Flow cytometry was applied to observe the change of cell proliferation cycle and apoptosis index.Cell morphological change was observed by transmission electron microscopy.Results COPADG inhibited effectively the growth of esophageal cancer cells Eca-109 and TE-1 in a time-and dose-dependent manner(P0.01).In Eca-109 and TE-1 cells treated by COPADG of different concentrations for 48 h,flow cytometry analysis showed that the percentage of G_(0)/G_(1)phase was higher and the percentage of S phase was lower than that in untreated cells(P0.01).A marked apoptosis peak was formed in Eca109 cells,and the typical apoptotic morphological change was observed by transmission electron microscopy.Conclusion COPADG can inhibit effectively the proliferation of esophageal cancer cells Eca-109 and TE-1,and it can change the distribution of cell cycle and induce apoptosis of tumor cells at certain concentrations.