Plant antigens with immunological properties. III. Separation of a biologically active fraction in a grass pollen extract

In present paper a partial biochemical study concerning two grass pollens (Poa pratensis and Phleum pratense) is carried out. On Sephadex G-75 three fractions were detected by an extract from the grass pollens. Only the third fraction shows a biological activity and biochemical findings are in agreement with the presence of four polipeptidic subfractions of different molecular weights ranged between 10,000 and 14,500. Specific additionals trials (ultracentrifugation, disc-electrophoresis, thyn-layer electrophoresis, isoelectric-focusing, immunological studies) are carried out to confirm the observed nature of prevalent material in pollens.