PURIFICATION OF FLUORESCENT CONJUGATES FROM FREE FLUOROCHROME WITH THE AID OF ION-EXCHANGE RESINS. REPORT 2. COMPARATIVE RESULTS OF FLUORESCENT ANTIBODY PURIFICATION BY THE METHODS OF ION EXCHANGE AND FILTRATION THROUGH GEL

Abstract : Conclusions: Purification of luminescent antibodies with the help of anion-exchange resin and sephadex produces practically the same results. A deficiency in purification with anion-exchange resins is connected with the necessity of the preliminary dialysis of the conjugates, which somewhat prolongs the time for purification; a deficiency of sephadex is its great expense and the considerable dilution of the solutions of purified antibodies. The extreme low price and availability of ion-exchange resins, and also their insignificant expenditure in work make it possible to recommend the latter, especially when it is necessary to purify significant amounts of luminescent antibodies, and this may have special importance under industrial conditions.