Introns Identification and Transient Expression Analysis of NtNAC-R1 Gene in Tobacco(Nicotiana tabacum L.)

Cloning and transient expression analysis of tobacco NtNAC-R1 gene was performed to discuss whether it contains introns and its function in nicotine biosynthesis.In this study,NAC transcription factor NtNAC-R1 was isolated by using tobacco genomic DNA and its two introns were identified.By constructing eukaryotic expression vector and establishing transient expression analysis system,over-expression of NtNAC-R1 gene followed by RT-PCR detection indicated that the expression amount of nicotine biosynthesis key gene PMT increased while JA signal marker gene PDF1.2 and IAA response gene IAA13 decreased.Transient expression analysis suggested that the gene regulated nicotine biosynthesis under the cross talking of JA and IAA signal pathway.