Detection of protein by the use of photoluminescence in rare earth-protein-SDBS system

The fluorescence enhancement effect in rare earth-protein-sodium dodecyl benzenesulfonate (SDBS) system has been studied. The photoluminescence of Tb3+-SDBS-protein system was very weak. However, when Gd3+or ethanol was added into this system, the fluorescence was significantly enhanced. In the case of collagen, this effect was greatest. Based on this, a method was established to determine proteins sensitively at nanogram/ml level. キー ワー ド=蛍 光、希土類元素イオン、タンパ ク質の検出