Physiological study of the use of bull seminal plasma in skim milk diluent to improve quality of frozen ram semen

Ram semen freezing process still generates poor outcome due to phospholipase. Thus, an alternative to substitute ram seminal plasma is recently developed using bull seminal plasma. The aim of this study was to determine the benefits of bull seminal plasma added by ram spermatozoa diluent in the freezing process. This study used control treatment (P0); (P1) was (diluent + ram semen) + bull seminal plasma (1: 1); (P2) was (diluent + ram semen) + bull seminal plasma (1:2). The result showed that P1 generated the highest viability, motility, plasma membrane intact, and the lowest DNA fragmentation, compared to second treatment and control. Statistical analysis of viability, motility, plasma membrane intact and DNA fragmentation were significantly different (p<0.05) among treatments. It can be concluded that additional of seminal plasma of bull can maintain motility, viability, plasma membrane intact and decrease the of DNA fragmentation in ram spermatozoa after-thawing.