Isolation ofNucleoli andLocalization ofRibonucleic Acid Polymerase IfromSoybean Hypocotyll
1975
An effective methodfortheisolation ofnucleoli from auxin-treated soybean(Glycine max,var.Wayne)hypocotyl was developed by polytronhiomogenization and sucrose gradient centrifugation. The nucleoli expressed onlythe a-amanitin-insensitive RNA synthetic activity. Thisactivitychromatographed asRNA polymerase IonDEAE-cellulose.Itappearsthattheplantnucleolus, liketheainimal nucleolus, isthesiteoflocalization forRNA polymerase I.
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