Effects of matrine on proliferation and telomerase activity of colon cancer SW1116 cells

Objective:To investigate the effects of matrine on proliferation and telomerase activity of colon cancer SW1116 cells. Methods:The proliferation inhibitory rate was evaluated by MTT assay. The telomerase activity was analyzed by TRAP-ELISA,and the expression of hTERT mRNA was determined by semi-quantitative RT-PCR. Results:Matrine displayed strong proliferation inhibitory effect in a dose-and-time-dependent manner against SW1116 cells. Compared with control group,the telomerase activity and the expression of hTERT mRNA decreased significantly(P0.05 or P0.01) in matrine group. Conclusion:Matrine can inhibit the telomerase activity by depressing the expression of hTERT in SW1116 cells and inhibiting cell proliferation.