Method of producing lily bulb by utilizing plant tissue culture technique

2013 
The invention provides a method of producing a lily bulb by utilizing plant tissue culture technique. Through three production steps of explants preparation, enrichment culture and bulb forming, the lily bulb production which is short in period, low in cost and simple to operate is finished. During the use process, an intermittent immersion plant bioreactor is used for the enrichment culture, the two steps of the enrichment culture and the bulb forming are finished in the same plant bioreactor, transfer operation on tissue culture seedlings is not needed in the culture process, and therefore a phase of adjustment occurring when the tissue culture seedlings are transferred to a new environment is avoided, and the production cycle is shortened. Only one time of inoculation is needed in the entire culture process, replacement of culture solution due to transfer operation on the tissue culture seedlings is avoided, the operations of frequent change over, filling cleaning of mass tissue culture bottles and the like are eliminated, the labor load is lightened greatly, and the production cost is lowered. Compared with traditional solid culture, the subculture frequency is reduced, and the aberration rate is lowered. Besides, culture is carried out in aseptic conditions, cross infection of virus is avoided, and the quality of the lily bulb is good.
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