Combination of liquid-phase pulsed discharge and ultrasonic for saponins extraction from lychee seeds.

Abstract A skillfully combined method of liquid-phase pulsed discharge and ultrasonic (LPDU) had been developed for saponins extraction from lychee seeds. Single factor and response surface methods were used to optimize the system, respectively. The optimized conditions included 30% aqueous ethanol, 62.66 mL/g ratio of liquid to solid, 3 mm centre hole diameter of hollow electrode, 123 mL/min flow velocity, length of serpentine pipe of 15 cm, 276 W ultrasonic power, 47 °C ultrasonic temperature, and discharge voltage was fixed at 14 kV. Under these conditions, it obtained a maximum saponins yield of 51.30 ± 0.08 mg/g with 10 min, which was higher than those of LPD (42.33 ± 0.98 mg/g) with 24 min, ultrasonic assisted extraction (UAE) (41.80 ± 1.31 mg/g) with 30 min and maceration (38.72 ± 1.13 mg/g) with 180 min. Meanwhile, the energy consumption of LPDU was 7560 kJ/kg, which was notably lower than those of LPD (8820 kJ/kg), UAE (25875 kJ/kg) and maceration (10248 kJ/kg). We found that the saponin constituents of LPDU were similar to LPD, UAE, ME by HPLC content detection method, and found that LPDU had the highest degree of tissue damage after scanning electron microscope (SEM) comparison, which verified the reason for its high extraction efficiency. The results showed that LPDU was an effective technology for saponins extraction, which may be potentially applied in cosmetics, medicines and food chemistry.