Synthesis and evaluation of a silica-bonded concanavalin A material for lectin affinity enrichment of N-linked glycoproteins and glycopeptides

A lectin affinity chromatography material was synthesized by covalent bonding of concanavalin A onto silica gel (named SG Con A) via a rapid, simple and robust method. The material exhibited high selectivity and efficiency as well as low sample loss for the selective enrichment of N-linked glycoproteins and glycopeptides.