Incorporation of glucocerebrosidase into Gaucher's disease

Several carriers were evaluated for use in the delivery of exogenous glucocerebrosidase to monocytes from Gaucher's disease patients. Only gamma globulin-coated, re- sealed erythrocytes proved to be an effective vehicle for enzyme delivery. Glucocerebrosidase added in this manner normalized intracellular enzyme levels for at least 18 hr. In this model sys- tem for the study of enzyme replacement therapy, soluble en- zyme, enzyme in uncoated resealed erythrocytes, and enzyme incorporated into liposomes were ineffective. Gaucher's disease is a glycolipid storage disorder characterized by the accumulation of glucocerebroside in tissue macrophages