Identification of sunfish species by muscle protein isoelectric focusing

Abstract 1. 1. Muscle protein phenotypes of nine recognized Lepomis species were developed by isoelectric focusing. 2. 2. Four classes were described based on putative parvalbumin patterns. 3. 3. All nine species could be distinguished by their protein phenotypes. 4. 4. Natural hybrids and their parentage were also identified, and the utility of this technique in studying natural sunfish hybridization is discussed.