Micropropagation of Aristolochia rotunda L.

AbstractAn original protocol of micropropagation of Aristolochia rotunda L., an important herb for the survival of an endangered butterfly was developed. The cytokinin 6-benzylaminopurine affected the production of new shoots but had no effect on shoot length. The addition of indole-3-butyric acid (IBA) had a negative effect on shoot length. Best results in terms of rooting percentage and root length were achieved with 1.5 μM IBA. The protocol can be employed to enhance the number of A. rotunda in the environment.