Tuberoside M, a new cytotoxic spirostanol saponin from the seeds of Allium tuberosum

Tuberoside M ( 1 ), isolated from the seeds of Allium tuberosum , shows a significant inhibitory effect on the growth of the human promyelocytic leukemia cell line (HL-60) with IC 50 value of 6.8 u w g/ml. On the basis of spectral data and chemical reaction, its structure was established as (25 S )-5 g; -spirostane-1 g; ,3 g; -diol 3- O - f; - l -rhamnopyranosyl-(1 M 4)- g; - d -glucopyranoside.