Manufacturing method of neural tissue

The present invention includes the following steps (1) to (3), to provide a method of manufacturing a neural cell or nerve tissue: (1) pluripotent stem cells, in absence of feeder cells, 1) TGF [beta family signaling pathway inhibitors and / or Sonic hedgehog signaling pathway agonist, and 2) culturing in a medium containing undifferentiated maintenance factor the first step of, (2) the cells obtained in the first step to suspension culture, second step, and (3) the resulting aggregate in the second step, the presence of a differentiation-inducing factor or non-forming aggregates of cells suspension cultured in the presence of a third step of obtaining an aggregate comprising a neural cell or neural tissue.