ONE-STEP AFFINITY PURIFICATION OF CYCLODEXTRIN GLUCANOTRANSFERASE FROM Bacillus SP. 1070

ligand, whereas the specic enzyme activity isolated on -CD-Sepharose 4B increased into 15 times (appr. 95% of purity in SDS Electrophoresis data). It was shown that the extent of starch conversion within crude enzyme did not exceed 70%, while with puried CGTase it was possible to get more than 95%.