[Cytotoxic effect of lappaconitine on non-small cell lung cancer in vitro and its molecular mechanism].

Objective: To evaluate the proliferation inhibitory effect of Lappaconitine(LAP) on non-small cell lung cancer cell line A549 cells in vitro and its possible mechanism.Methods: A549 cell was cultured with different concentrations of LAP.Cellular proliferation was determined with MTT.Cell cycle and apoptosis were detected with FCM technology.The Cyclin E1 gene expression was checked by Real-time Quantitative PCR method.Results: LAP could inhibit the proliferation of A549 cells in vitro in a dose-dependent manner.LAP could induce apoptosis of A549 cell.Cell cycle was stopped at the G1+ G0phase by LAP with FCM technology.With the increasement of LAP concentration,the ratio of G1+ G0phase was increased and the ratio of S phase and G2+ M phase was decreased;The apoptotic rate was gradually increased,and the Cyclin E1 gene expression was down-regulated.Conclusion: LAP has the inhibitory effect on the growth of A549 cells,which is related to the cell cycle arrest in G0/G1phase,apoptosis and down-regulation of Cyclin E1gene expression.