Application of nano-gold directly bonded with luminol in immunoassay

The present invention discloses the use of luminol directly bonded gold nanoparticles in immunoassays. One gold nanoparticles immunoassay probe is directly bonded luminol, consisting of the analysis of gold nanoparticles labeled probe luminol directly bonded by the antibody. Wherein luminol directly bonded is a step of gold nanoparticles by reduction of chloroauric acid obtained luminol. The second method is a chemiluminescence immunoassay luminol directly bonded to the gold nanoparticle probe-based immunoassay. The third embodiment is a kit for the analytical method. Chemiluminescence immunoassay method of the present invention has high sensitivity (as determined in a human IgG detection limit of 1.0pg / mL), wide linear range, reproducible, simple operation, low cost, etc., can be used in various samples Determination of the analytes. It has important applications in clinical diagnosis and treatment, drug analysis, food safety testing, environmental monitoring and other fields.