Biochemical metabolism of two cultivars of cowpea treated with 24-Epibrassinolide and subjected to saline stress

We aimed to evaluate the changes in biochemical metabolism generated by salt stress and to investigate the effect of brassinosteroids in mitigating of this stress on two cultivars of Vigna unguiculata L. We used a completely randomized experimental design in a 2 x 3 x 3 factorial scheme, using two cultivars of cowpea (BRS Guariba and BR3 Tracuateua - moderately tolerant and sensitive to salinity, respectively), three concentrations of brassinosteroids (0, 0.2 and 0.4 muM Br) and three concentrations of NaCl (0 , 50 and 100 mM NaCl), with four replicates. The following evaluations were carried out: relative water content, electrolyte leakage, nitrate levels, nitrate reductase activity, free ammonium, total soluble amino acids, soluble proteins, glycine betaine and proline. The results showed that salinity at 100 mM affected the nitrate reductase enzyme activity, the relative water content, total soluble amino acids and soluble proteins for cultivars BR3 Tracuateua, and BRS Guariba, but the 24-epibrassinolid attenuated the effects of salinity for these variables. The concentration of 0.2 muM of Br increased 55% and 20% in proline and glycine betaine contents, respectively, in both of cultivars. The plants under stress saline and 0.2 mM of 24-epibrassinolid, presented 42% and 58% reductions in electrolyte leakage of BR3 Tracuateua and BRS Guariba cultivars, respectively. The concentrations of ammonium were slightly varied. Therefore, the application of 0.2 muM of 24-epibrassinolid caused a greater acclimatization of the cultivars, being the BR3 Tracuateua (sensitive to salt) cultivar more expressive in most treatments.